General Cell Based Assays
Cayman Chemical Catlase asy KIt 96Wel
Catalase is an ubiquitous antioxidant enzyme that is present in most aerobic cells. It is involved in the detoxification of H2O2, a ROS that is a toxic product of both normal aerobic metabolism and pathogenic ROS production. Cayman’s Catalase Assay utilizes the peroxidatic function of catalase for determination of enzyme activity. The assay can be used to measure catalase activity in plasma, serum, erythrocyte lysates, tissue homogenates, and cell lysates.
Enzo Life Sciences Red hydrogen peroxide assay kit (1Kit)
Red Hydrogen Peroxide Assay Kit provides a simple absorbance-based or fluorometric assay to detect and quantify hydrogen peroxide or peroxidase activity in biochemical assays, cell extracts and in live cells. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions. The kit is an optimized ‘mix and read’ assay that is suitable for both 96- and 348-well formats and is compatible with HTS liquid handling. Applications: Fluorescence microscopy, HTS .
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-PLC-γ1 (Tyr783) Detection Kit, 50,000 Assay Points
HTRF Human & Mouse Phospho-PLC-γ1 (Tyr783) Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total MKK4 Detection Kit, 500 Assay Points
HTRF Human Total MKK4 Detection Kit, 500 Assay Points
Cell Biolabs Inc NAD+/NADH Assay Kit (Colorimetric), 100 assays
Cell Biolabs’ NAD+/NADH Assay Kit (Colorimetric) is a simple assay that measures NAD+ and NADH present in biological samples such as cell lysates or tissue extracts in a 96-well microtiter plate format. The kit is specific for NAD+, NADH, and their ratio. The kit will not detect NADP+ or NADPH. The total NAD+/NADH concentrations of unknown samples are determined by comparison with a known NAD+ standard. Determination of both NAD+ and NADH requires two separate samples for quantification. NAD+ and NADH do not need to be purified from samples, but rather can be extracted individually with a simple acid or base treatment prior to performing the assay. The kit has a detection sensitivity limit of approximately 4 nM NAD+. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, NAD+ standards and unknown samples.
Cell Biolabs Inc DAG (Diacylglyercol) Assay Kit, 100 assays
Diacylglycerols (DAG) are key intermediates in the biosynthesis of many cellular lipids and play a fundamental role in biochemical signaling. Cell Biolabs’ DAG Assay Kit simple 96-well fluorometric assay that measures diacylglycerol content in samples by a coupled enzymatic reaction system. First, kinase is used to phosphorylate DAG samples, yielding phosphatidic acid. Next, a lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. Finally, the glycerol-3-phosphate product is oxidized by glycerol-3-phosphate oxidase (GPO), producing hydrogen peroxide which reacts with the kit’s fluorometric probe. The kit contains a DAG Standard and has a detection sensitivity limit of 15 µM. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, standards and unknown samples. Note each sample replicate requires 2 assays, one of which is a negative control to be subtracted as background.
Bioassay Systems EnzyChrom™ L-Lactate Assay Kit. For quantitative determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism.
For quantitative determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM L-Lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM L-Lactate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Serum, plasma, cell culture media etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.05 mM.
Bioassay Systems EnzyChrom™ Nitric Oxide Synthase Assay Kit. For quantitative determination of nitric oxide synthase (NOS) activity and screen for NOS inhibitor.
For quantitative determination of nitric oxide synthase (NOS) activity and screen for NOS inhibitor. Key Features: Sensitive and accurate. Detection range 0.25 - 25 U/L in 96-well plate. Rapid and reliable. Can be completed in 40 min if reduction of NO3- to NO2- is performed at 60°C. Method: OD540nm. Samples: Biological. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.25 U/L.
Bioassay Systems EnzyChrom™ Cholesterol Assay Kit. For quantitative determination of cholesterol and evaluation of drug effects on cholesterol metabolism.
For quantitative determination of cholesterol and evaluation of drug effects on cholesterol metabolism. Key Features: Sensitive and accurate. Detection limit of 5 mg/dL, linearity up to 300 mg/dL cholesterol in 96-well plate assay. Convenient. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD340nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 5 mg/dL.
Revvity Health Sciences Inc AlphaLISA High Performance Biotin-Free Human TNFα Detection Kit, 100 Assay Points
AlphaLISA High Performance Biotin-Free Human TNFα Detection Kit, 100 Assay Points
Revvity Health Sciences Inc AlphaScreen HA Detection Kit, 50,000 Assay Points
AlphaScreen HA Detection Kit, 50,000 Assay Points